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Adaptation of fluorescent technique for genotyping with new microsatellite markers in common bean PAB
Oblessuc,Paula Rodrigues; Campos,Tatiana de; Cardoso,Juliana Morini Kupper; Sforça,Danilo Augusto; Baroni,Renata Moro; Souza,Anete Pereira de; Benchimol,Luciana Lasry.
Tipo: Info:eu-repo/semantics/article
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000600013
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Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers Scientia Agricola
Sousa,Adna Cristina Barbosa de; Godoy,Rodolfo; Sforça,Danilo Augusto; Campos,Tatiana de; Zucchi,Maria Imaculada; Jank,Liana; Souza,Anete Pereira de.
The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legumes; Transferability; Microsatellite; Germplasm.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007
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New microsatellite markers developed from an enriched microsatellite common bean library PAB
Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry.
The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019
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Potato cultivar identification using molecular markers PAB
Rosa,Patricia Marchezi; Campos,Tatiana de; Sousa,Adna Cristina Barbosa de; Sforça,Danilo Augusto; Torres,Gisele Abigail Montan; Souza,Anete Pereira de.
The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum; AFLP; Genetic certification; Varietal protection; Variety identification; RAPD.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000100015
Registros recuperados: 4
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